Md. Sagir Ahmed, Nafisa Nawal Islam, JBM Aysha Akter and Nusrat Jahan Sanzida

DNA barcoding of Smiliogastrinae (Teleostei: Cypriniformes) of Bangladesh based on cytochrome c oxidase subunit I (COI) sequences

Abstract This study aims to molecularly characterize the phylogenetic relationship of small barbs under the Subfamily Smiliogastrinae (Cypriniformes: Cyprinidae) in Bangladesh using a fragment of the mitochondrial gene cytochrome c oxidase subunit I (COI). Samples were collected from rivers, haors (a seasonal wetland), baors (an oxbow lake), beels (perennial waterbody), and floodplains. A total of eleven species under five genera were confirmed based on both morphological and molecular approaches. The average Kimura two-parameter (K2P) distances for intraspecies and interspecies were 0.0058 and 0.1538, respectively. The mean GC content was markedly low (44.03%) in the COI sequences of the smiliogastrin species compared to the mean AT content (55.97%). In addition to the barcode-based species identification, phylogenetic relationships among the species were also explored. Phylogenetic (neighbor-joining, parsimony, and maximum likelihood) as well as species delimitation (ASAP and mPTP) analyses of all the eleven species revealed distinct clusters in concurrence with the taxonomic status of the species.


Keywords Smiliogastrinae; Barbs; COI; Cyprinidae; DNA barcoding; Phylogeny

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